The Comparison of Hemoglobin and Leukocytes Examination Between Whole Blood and Pre-Diluted Modes on Hematology Analyzer
Keywords:
Hemoglobin, hematology analyzer, leukocytes, pre-diluted, whole bloodAbstract
Hematological testing plays a vital role in assessing the condition of blood and its components, such as hemoglobin and leukocytes. One of the commonly used methods is the automatic hematology analyzer, which operates with two measurement modes: whole blood for standard sample volumes (±1 mL) and pre-diluted for non-standard volumes (>20 µL to <1 mL), typically due to pre-analytical factors. This study aims to compare hemoglobin levels and leukocyte counts between the whole blood and pre-diluted modes. The study applied an observational analytic study with a cross-sectional design involving 30 randomly blood samples. Each sample was analyzed using both modes, and the results were statistically evaluated using a paired t-test. The findings revealed a significant difference in hemoglobin levels between the whole blood and pre-diluted modes (p = 0.002), while no significant difference was observed in leukocyte counts (p = 0.40). Although the difference in hemoglobin levels was statistically significant, it was not clinically relevant, and both modes obtained results that were acceptable for clinical interpretation.
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