Isolation & Antagonistic Test Of Soil Bacteria Againstm Rhizoctonia Sp. A Pathogen Of Rice Plant (Oryza Sativa L.)

Abstract

Purpose:

This study aims to isolate bacteria from soil that are capable of producing antimicrobial compounds, as well as to test the inhibitory activity against the growth of Rhizoctonia sp.

Method:

Soil samples were taken at 3 locations of  PT Biotek Cipta Kreasi land, then cultured in nutrient agar media with stratified dilutions, then observations of colony morphology and cell morphology were made, then antagonistic tests were carried out in vitro and observations were made.

Results:

The results showed that there were 23 isolates of soil bacteria with various characteristics and shapes. Then 17 gram-positive and rod-shaped bacteria were tested for antagonism, showing that the 17 bacteria did not have inhibition against the growth of Rhizoctonia sp.